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One-step hot formamide extraction of RNA from Saccharomyces cerevisiae
Current methods for isolating RNA from budding yeast require lengthy and laborious steps such as freezing and heating with phenol, homogenization with glass beads, or enzymatic digestion of the cell wall. Here, extraction with a solution of formamide and EDTA was adapted to isolate RNA from whole ye...
Autores principales: | Shedlovskiy, Daniel, Shcherbik, Natalia, Pestov, Dimitri G. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731811/ https://www.ncbi.nlm.nih.gov/pubmed/28692404 http://dx.doi.org/10.1080/15476286.2017.1345417 |
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