Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding

Amplicon based metabarcoding promises rapid and cost-efficient analyses of species composition. However, it is disputed whether abundance estimates can be derived from metabarcoding due to taxon specific PCR amplification biases. PCR-free approaches have been suggested to mitigate this problem, but...

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Detalles Bibliográficos
Autores principales: Krehenwinkel, Henrik, Wolf, Madeline, Lim, Jun Ying, Rominger, Andrew J., Simison, Warren B., Gillespie, Rosemary G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732254/
https://www.ncbi.nlm.nih.gov/pubmed/29247210
http://dx.doi.org/10.1038/s41598-017-17333-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732254/
https://www.ncbi.nlm.nih.gov/pubmed/29247210
http://dx.doi.org/10.1038/s41598-017-17333-x

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