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Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding

Amplicon based metabarcoding promises rapid and cost-efficient analyses of species composition. However, it is disputed whether abundance estimates can be derived from metabarcoding due to taxon specific PCR amplification biases. PCR-free approaches have been suggested to mitigate this problem, but...

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Detalles Bibliográficos
Autores principales:	Krehenwinkel, Henrik, Wolf, Madeline, Lim, Jun Ying, Rominger, Andrew J., Simison, Warren B., Gillespie, Rosemary G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732254/ https://www.ncbi.nlm.nih.gov/pubmed/29247210 http://dx.doi.org/10.1038/s41598-017-17333-x

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