The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli

BACKGROUND: To optimize the production of membrane and secretory proteins in Escherichia coli, it is critical to harmonize the expression rates of the genes encoding these proteins with the capacity of their biogenesis machineries. Therefore, we engineered the Lemo21(DE3) strain, which is derived fr...

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Detalles Bibliográficos
Autores principales: Kuipers, Grietje, Karyolaimos, Alexandros, Zhang, Zhe, Ismail, Nurzian, Trinco, Gianluca, Vikström, David, Slotboom, Dirk Jan, de Gier, Jan-Willem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732464/
https://www.ncbi.nlm.nih.gov/pubmed/29246156
http://dx.doi.org/10.1186/s12934-017-0840-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732464/
https://www.ncbi.nlm.nih.gov/pubmed/29246156
http://dx.doi.org/10.1186/s12934-017-0840-4

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