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Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit

The recent adaptation of CRISPR Cas9 genome editing to Leishmania spp. has opened a new era in deciphering Leishmania biology. The method was recently improved using a PCR-based CRISPR Cas9 approach, which eliminated the need for cloning. This new approach, which allows high-throughput gene deletion...

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Detalles Bibliográficos
Autores principales: Martel, Daniel, Beneke, Tom, Gluenz, Eva, Späth, Gerald F., Rachidi, Najma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733176/
https://www.ncbi.nlm.nih.gov/pubmed/29333442
http://dx.doi.org/10.1155/2017/4635605