Cargando…

A Method for the Acute and Rapid Degradation of Endogenous Proteins

Methods for the targeted disruption of protein function have revolutionized science and greatly expedited the systematic characterization of genes. Two main approaches are currently used to disrupt protein function: DNA knockout and RNA interference, which act at the genome and mRNA level, respectiv...

Descripción completa

Detalles Bibliográficos
Autores principales:	Clift, Dean, McEwan, William A., Labzin, Larisa I., Konieczny, Vera, Mogessie, Binyam, James, Leo C., Schuh, Melina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cell Press 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733393/ https://www.ncbi.nlm.nih.gov/pubmed/29153837 http://dx.doi.org/10.1016/j.cell.2017.10.033

Ejemplares similares

Two mechanisms drive pronuclear migration in mouse zygotes
por: Scheffler, Kathleen, et al.
Publicado: (2021)

A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes
por: Clift, Dean, et al.
Publicado: (2015)

Antibody and DNA sensing pathways converge to activate the inflammasome during primary human macrophage infection
por: Labzin, Larisa I, et al.
Publicado: (2019)

Seeing is believing: Representation as a powerful tool in the fight against racism in science
por: Mogessie, Binyam
Publicado: (2021)

Actin limits egg aneuploidies associated with female reproductive aging
por: Dunkley, Sam, et al.
Publicado: (2023)

Spire and Formin 2 Synergize and Antagonize in Regulating Actin Assembly in Meiosis by a Ping-Pong Mechanism
por: Montaville, Pierre, et al.
Publicado: (2014)

A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation
por: Mogessie, Binyam, et al.
Publicado: (2015)

The prophase oocyte nucleus is a homeostatic G-actin buffer
por: Scheffler, Kathleen, et al.
Publicado: (2022)

Stoking inflammasome fires in the COVID-19 neighborhood
por: Labzin, Larisa I., et al.
Publicado: (2023)

Inflammatory cell death: how macrophages sense neighbouring cell infection and damage
por: Wang, Xiaohui, et al.
Publicado: (2023)

Responding to rapid change in libraries: a user experience approach
por: McEwan, Jessica
Publicado: (2022)

Rapid and reversible knockdown of endogenous proteins by peptide-directed lysosomal degradation
por: Fan, Xuelai, et al.
Publicado: (2014)

A Contrast in Methods of Vaccination: By Intradermal Injection and by Linear Scratch
por: McEwan, Tom
Publicado: (1940)

Trim-Away ubiquitinates and degrades lysine-less and N-terminally acetylated substrates
por: Kiss, Leo, et al.
Publicado: (2023)

Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons
por: Macdonald, Lewis, et al.
Publicado: (2022)

Rapid and Reversible Knockdown of Endogenously Tagged Endosomal Proteins via an Optimized HaloPROTAC Degrader
por: Tovell, Hannah, et al.
Publicado: (2019)

RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination
por: Kiss, Leo, et al.
Publicado: (2021)

Advances in targeted degradation of endogenous proteins
por: Röth, Sascha, et al.
Publicado: (2019)

Detection and destruction of antibody-bound viruses by TRIM21
por: McEwan, William
Publicado: (2013)

Dysregulated Inflammation During Obesity: Driving Disease Severity in Influenza Virus and SARS-CoV-2 Infections
por: Hulme, Katina D., et al.
Publicado: (2021)

ARF‐AID: A Rapidly Inducible Protein Degradation System That Preserves Basal Endogenous Protein Levels
por: Sathyan, Kizhakke Mattada, et al.
Publicado: (2020)

A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets
por: Layden, Hillary M., et al.
Publicado: (2021)

Study on Rapid Detection Method for Degradation Performance of Polyolefin-Based Degradable Plastics
por: Zhou, Jinglun, et al.
Publicado: (2022)

Surveillance for Intracellular Antibody by Cytosolic Fc Receptor TRIM21
por: McEwan, William A.
Publicado: (2016)

Antibodies in the intracellular domain
por: McEwan, William A.
Publicado: (2022)

Ovine rumen papillae biopsy via oral endoscopy; a rapid and repeatable method for serial sampling
por: McRae, KM, et al.
Publicado: (2016)

A novel and robust method for counting components within bio-molecular complexes using fluorescence microscopy and statistical modelling
por: Mersmann, Sophia F., et al.
Publicado: (2022)

TRIM21 Promotes cGAS and RIG-I Sensing of Viral Genomes during Infection by Antibody-Opsonized Virus
por: Watkinson, Ruth E., et al.
Publicado: (2015)

Exclusion and Genomic Relatedness Methods for Assignment of Parentage Using Genotyping-by-Sequencing Data
por: Dodds, Ken G., et al.
Publicado: (2019)

Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis
por: Castro-Dopico, Tomas, et al.
Publicado: (2019)

Rapid Standardized CT-Based Method to Determine Lean Body Mass SUV for PET—A Significant Improvement Over Prediction Equations
por: Riauka, Terence A., et al.
Publicado: (2022)

Unassembled CD147 is an endogenous endoplasmic reticulum–associated degradation substrate
por: Tyler, Ryan E., et al.
Publicado: (2012)

Pathogen-driven degradation of endogenous and therapeutic antibodies during streptococcal infections
por: Toledo, Alejandro Gomez, et al.
Publicado: (2023)

A TRIM21-based bioPROTAC highlights the therapeutic benefit of HuR degradation
por: Fletcher, Alice, et al.
Publicado: (2023)

Rapid endogenic rock recycling in magmatic arcs
por: Li, Jun-Yong, et al.
Publicado: (2021)

HIV-1 uses dynamic capsid pores to import nucleotides and fuel encapsidated DNA synthesis
por: Jacques, David A., et al.
Publicado: (2016)

IRE1α is an endogenous substrate of endoplasmic reticulum-associated degradation
por: Sun, Shengyi, et al.
Publicado: (2015)

5th International Workshop on Next-generation Linear Colliders
por: Asher, Kathy, et al.
Publicado: (1993)

Tenascin-C fragments are endogenous inducers of cartilage matrix degradation
por: Sofat, Nidhi, et al.
Publicado: (2011)

Rapid Liquid Chromatographic Method for the Determination of Roflumilast in the Presence of Degradation Products
por: Barhate, V D, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_jn8mfjpcl2sbv2q5teqql5jgup