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Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner

RNA-binding protein Sbp1 facilitates the decapping pathway in mRNA metabolism and inhibits global mRNA translation by an unclear mechanism. Here we report molecular interactions responsible for Sbp1-mediated translation inhibition of mRNA encoding the polyadenosine-binding protein (Pab1), an essenti...

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Detalles Bibliográficos
Autores principales: Brandariz-Núñez, Alberto, Zeng, Fuxing, Lam, Quan Ngoc, Jin, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733569/
https://www.ncbi.nlm.nih.gov/pubmed/28986506
http://dx.doi.org/10.1261/rna.062547.117
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author Brandariz-Núñez, Alberto
Zeng, Fuxing
Lam, Quan Ngoc
Jin, Hong
author_facet Brandariz-Núñez, Alberto
Zeng, Fuxing
Lam, Quan Ngoc
Jin, Hong
author_sort Brandariz-Núñez, Alberto
collection PubMed
description RNA-binding protein Sbp1 facilitates the decapping pathway in mRNA metabolism and inhibits global mRNA translation by an unclear mechanism. Here we report molecular interactions responsible for Sbp1-mediated translation inhibition of mRNA encoding the polyadenosine-binding protein (Pab1), an essential translation factor that stimulates mRNA translation and inhibits mRNA decapping in eukaryotic cells. We demonstrate that the two distal RRMs of Sbp1 bind to the poly(A) sequence in the 5′UTR of the Pab1 mRNA specifically and cooperatively while the central RGG domain of the protein interacts directly with Pab1. Furthermore, methylation of arginines in the RGG domain abolishes the protein–protein interaction and the inhibitory effect of Sbp1 on translation initiation of Pab1 mRNA. Based on these results, the underlying mechanism for Sbp1-specific translational regulation is proposed. The functional differences of Sbp1 and RGG repeats alone on transcript-specific translation were observed, and a comparison of the results suggests the importance of remodeling the 5′UTR by RNA-binding proteins in mRNA translation.
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spelling pubmed-57335692018-01-01 Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner Brandariz-Núñez, Alberto Zeng, Fuxing Lam, Quan Ngoc Jin, Hong RNA Article RNA-binding protein Sbp1 facilitates the decapping pathway in mRNA metabolism and inhibits global mRNA translation by an unclear mechanism. Here we report molecular interactions responsible for Sbp1-mediated translation inhibition of mRNA encoding the polyadenosine-binding protein (Pab1), an essential translation factor that stimulates mRNA translation and inhibits mRNA decapping in eukaryotic cells. We demonstrate that the two distal RRMs of Sbp1 bind to the poly(A) sequence in the 5′UTR of the Pab1 mRNA specifically and cooperatively while the central RGG domain of the protein interacts directly with Pab1. Furthermore, methylation of arginines in the RGG domain abolishes the protein–protein interaction and the inhibitory effect of Sbp1 on translation initiation of Pab1 mRNA. Based on these results, the underlying mechanism for Sbp1-specific translational regulation is proposed. The functional differences of Sbp1 and RGG repeats alone on transcript-specific translation were observed, and a comparison of the results suggests the importance of remodeling the 5′UTR by RNA-binding proteins in mRNA translation. Cold Spring Harbor Laboratory Press 2018-01 /pmc/articles/PMC5733569/ /pubmed/28986506 http://dx.doi.org/10.1261/rna.062547.117 Text en © 2018 Brandariz-Núñez et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Brandariz-Núñez, Alberto
Zeng, Fuxing
Lam, Quan Ngoc
Jin, Hong
Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner
title Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner
title_full Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner
title_fullStr Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner
title_full_unstemmed Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner
title_short Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner
title_sort sbp1 modulates the translation of pab1 mrna in a poly(a)- and rgg-dependent manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733569/
https://www.ncbi.nlm.nih.gov/pubmed/28986506
http://dx.doi.org/10.1261/rna.062547.117
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