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Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv

CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E. coli in the presence and absence of CyDisCo, with >90% being solub...

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Detalles Bibliográficos
Autores principales: Gąciarz, Anna, Ruddock, Lloyd W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5734687/
https://www.ncbi.nlm.nih.gov/pubmed/29253024
http://dx.doi.org/10.1371/journal.pone.0189964