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Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv
CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E. coli in the presence and absence of CyDisCo, with >90% being solub...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5734687/ https://www.ncbi.nlm.nih.gov/pubmed/29253024 http://dx.doi.org/10.1371/journal.pone.0189964 |