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Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels

The self-assembled bilayer lipid membrane (BLM) is the basic component of the cell membrane. The reconstitution of ion channel proteins in artificially formed BLMs represents a well-defined system for the functional analysis of ion channels and screening the effects of drugs that act on them. Howeve...

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Autores principales: Tadaki, Daisuke, Yamaura, Daichi, Araki, Shun, Yoshida, Miyu, Arata, Kohei, Ohori, Takeshi, Ishibashi, Ken-ichi, Kato, Miki, Ma, Teng, Miyata, Ryusuke, Tozawa, Yuzuru, Yamamoto, Hideaki, Niwano, Michio, Hirano-Iwata, Ayumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735097/
https://www.ncbi.nlm.nih.gov/pubmed/29255199
http://dx.doi.org/10.1038/s41598-017-17905-x
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author Tadaki, Daisuke
Yamaura, Daichi
Araki, Shun
Yoshida, Miyu
Arata, Kohei
Ohori, Takeshi
Ishibashi, Ken-ichi
Kato, Miki
Ma, Teng
Miyata, Ryusuke
Tozawa, Yuzuru
Yamamoto, Hideaki
Niwano, Michio
Hirano-Iwata, Ayumi
author_facet Tadaki, Daisuke
Yamaura, Daichi
Araki, Shun
Yoshida, Miyu
Arata, Kohei
Ohori, Takeshi
Ishibashi, Ken-ichi
Kato, Miki
Ma, Teng
Miyata, Ryusuke
Tozawa, Yuzuru
Yamamoto, Hideaki
Niwano, Michio
Hirano-Iwata, Ayumi
author_sort Tadaki, Daisuke
collection PubMed
description The self-assembled bilayer lipid membrane (BLM) is the basic component of the cell membrane. The reconstitution of ion channel proteins in artificially formed BLMs represents a well-defined system for the functional analysis of ion channels and screening the effects of drugs that act on them. However, because BLMs are unstable, this limits the experimental throughput of BLM reconstitution systems. Here we report on the formation of mechanically stable solvent-free BLMs in microfabricated apertures with defined nano- and micro-tapered edge structures. The role of such nano- and micro-tapered structures on the stability of the BLMs was also investigated. Finally, this BLM system was combined with a cell-free synthesized human ether-a-go-go-related gene channel, a cardiac potassium channel whose relation to arrhythmic side effects following drug treatment is well recognized. Such stable BLMs as these, when combined with a cell-free system, represent a potential platform for screening the effects of drugs that act on various ion-channel genotypes.
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spelling pubmed-57350972017-12-21 Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels Tadaki, Daisuke Yamaura, Daichi Araki, Shun Yoshida, Miyu Arata, Kohei Ohori, Takeshi Ishibashi, Ken-ichi Kato, Miki Ma, Teng Miyata, Ryusuke Tozawa, Yuzuru Yamamoto, Hideaki Niwano, Michio Hirano-Iwata, Ayumi Sci Rep Article The self-assembled bilayer lipid membrane (BLM) is the basic component of the cell membrane. The reconstitution of ion channel proteins in artificially formed BLMs represents a well-defined system for the functional analysis of ion channels and screening the effects of drugs that act on them. However, because BLMs are unstable, this limits the experimental throughput of BLM reconstitution systems. Here we report on the formation of mechanically stable solvent-free BLMs in microfabricated apertures with defined nano- and micro-tapered edge structures. The role of such nano- and micro-tapered structures on the stability of the BLMs was also investigated. Finally, this BLM system was combined with a cell-free synthesized human ether-a-go-go-related gene channel, a cardiac potassium channel whose relation to arrhythmic side effects following drug treatment is well recognized. Such stable BLMs as these, when combined with a cell-free system, represent a potential platform for screening the effects of drugs that act on various ion-channel genotypes. Nature Publishing Group UK 2017-12-18 /pmc/articles/PMC5735097/ /pubmed/29255199 http://dx.doi.org/10.1038/s41598-017-17905-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tadaki, Daisuke
Yamaura, Daichi
Araki, Shun
Yoshida, Miyu
Arata, Kohei
Ohori, Takeshi
Ishibashi, Ken-ichi
Kato, Miki
Ma, Teng
Miyata, Ryusuke
Tozawa, Yuzuru
Yamamoto, Hideaki
Niwano, Michio
Hirano-Iwata, Ayumi
Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
title Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
title_full Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
title_fullStr Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
title_full_unstemmed Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
title_short Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
title_sort mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized herg channels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735097/
https://www.ncbi.nlm.nih.gov/pubmed/29255199
http://dx.doi.org/10.1038/s41598-017-17905-x
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