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Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels
The self-assembled bilayer lipid membrane (BLM) is the basic component of the cell membrane. The reconstitution of ion channel proteins in artificially formed BLMs represents a well-defined system for the functional analysis of ion channels and screening the effects of drugs that act on them. Howeve...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735097/ https://www.ncbi.nlm.nih.gov/pubmed/29255199 http://dx.doi.org/10.1038/s41598-017-17905-x |
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author | Tadaki, Daisuke Yamaura, Daichi Araki, Shun Yoshida, Miyu Arata, Kohei Ohori, Takeshi Ishibashi, Ken-ichi Kato, Miki Ma, Teng Miyata, Ryusuke Tozawa, Yuzuru Yamamoto, Hideaki Niwano, Michio Hirano-Iwata, Ayumi |
author_facet | Tadaki, Daisuke Yamaura, Daichi Araki, Shun Yoshida, Miyu Arata, Kohei Ohori, Takeshi Ishibashi, Ken-ichi Kato, Miki Ma, Teng Miyata, Ryusuke Tozawa, Yuzuru Yamamoto, Hideaki Niwano, Michio Hirano-Iwata, Ayumi |
author_sort | Tadaki, Daisuke |
collection | PubMed |
description | The self-assembled bilayer lipid membrane (BLM) is the basic component of the cell membrane. The reconstitution of ion channel proteins in artificially formed BLMs represents a well-defined system for the functional analysis of ion channels and screening the effects of drugs that act on them. However, because BLMs are unstable, this limits the experimental throughput of BLM reconstitution systems. Here we report on the formation of mechanically stable solvent-free BLMs in microfabricated apertures with defined nano- and micro-tapered edge structures. The role of such nano- and micro-tapered structures on the stability of the BLMs was also investigated. Finally, this BLM system was combined with a cell-free synthesized human ether-a-go-go-related gene channel, a cardiac potassium channel whose relation to arrhythmic side effects following drug treatment is well recognized. Such stable BLMs as these, when combined with a cell-free system, represent a potential platform for screening the effects of drugs that act on various ion-channel genotypes. |
format | Online Article Text |
id | pubmed-5735097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57350972017-12-21 Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels Tadaki, Daisuke Yamaura, Daichi Araki, Shun Yoshida, Miyu Arata, Kohei Ohori, Takeshi Ishibashi, Ken-ichi Kato, Miki Ma, Teng Miyata, Ryusuke Tozawa, Yuzuru Yamamoto, Hideaki Niwano, Michio Hirano-Iwata, Ayumi Sci Rep Article The self-assembled bilayer lipid membrane (BLM) is the basic component of the cell membrane. The reconstitution of ion channel proteins in artificially formed BLMs represents a well-defined system for the functional analysis of ion channels and screening the effects of drugs that act on them. However, because BLMs are unstable, this limits the experimental throughput of BLM reconstitution systems. Here we report on the formation of mechanically stable solvent-free BLMs in microfabricated apertures with defined nano- and micro-tapered edge structures. The role of such nano- and micro-tapered structures on the stability of the BLMs was also investigated. Finally, this BLM system was combined with a cell-free synthesized human ether-a-go-go-related gene channel, a cardiac potassium channel whose relation to arrhythmic side effects following drug treatment is well recognized. Such stable BLMs as these, when combined with a cell-free system, represent a potential platform for screening the effects of drugs that act on various ion-channel genotypes. Nature Publishing Group UK 2017-12-18 /pmc/articles/PMC5735097/ /pubmed/29255199 http://dx.doi.org/10.1038/s41598-017-17905-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tadaki, Daisuke Yamaura, Daichi Araki, Shun Yoshida, Miyu Arata, Kohei Ohori, Takeshi Ishibashi, Ken-ichi Kato, Miki Ma, Teng Miyata, Ryusuke Tozawa, Yuzuru Yamamoto, Hideaki Niwano, Michio Hirano-Iwata, Ayumi Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels |
title | Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels |
title_full | Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels |
title_fullStr | Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels |
title_full_unstemmed | Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels |
title_short | Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels |
title_sort | mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized herg channels |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735097/ https://www.ncbi.nlm.nih.gov/pubmed/29255199 http://dx.doi.org/10.1038/s41598-017-17905-x |
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