Dual direction CRISPR transcriptional regulation screening uncovers gene networks driving drug resistance

Pooled CRISPR–Cas9 knock out screens provide a valuable addition to the methods available for novel drug target identification and validation. However, where gene editing is targeted to amplified loci, the resulting multiple DNA cleavage events can be a cause of false positive hit identification. Th...

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Detalles Bibliográficos
Autores principales: le Sage, Carlos, Lawo, Steffen, Panicker, Prince, Scales, Tim M. E., Rahman, Syed Asad, Little, Annette S., McCarthy, Nicola J., Moore, Jonathan D., Cross, Benedict C. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735189/
https://www.ncbi.nlm.nih.gov/pubmed/29255251
http://dx.doi.org/10.1038/s41598-017-18172-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735189/
https://www.ncbi.nlm.nih.gov/pubmed/29255251
http://dx.doi.org/10.1038/s41598-017-18172-6

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