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Comparison of commercial RNA extraction kits and qPCR master mixes for studying gene expression in small biopsy tissue samples from the equine gastric epithelium

Gastric tissue biopsy and gene expression analysis are important tools for disease diagnosis and study of the physiology of the equine stomach. However, RNA extraction from gastric biopsy samples is a complex procedure because the samples contain low quantities of RNA and are contaminated with mucou...

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Detalles Bibliográficos
Autores principales: TESENA, Parichart, KORCHUNJIT, Wasamon, TAYLOR, Jane, WONGTAWAN, Tuempong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Equine Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735310/
https://www.ncbi.nlm.nih.gov/pubmed/29270070
http://dx.doi.org/10.1294/jes.28.135