Total internal reflection fluorescence anisotropy imaging microscopy: setup, calibration, and data processing for protein polymerization measurements in living cells

Fluorescence anisotropy imaging microscopy (FAIM) measures the depolarization properties of fluorophores to deduce molecular changes in their environment. For successful FAIM, several design principles have to be considered and a thorough system-specific calibration protocol is paramount. One import...

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Detalles Bibliográficos
Autores principales: Ströhl, Florian, Wong, Hovy H W, Holt, Christine E, Kaminski, Clemens F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOP Publishing 2018
Materias:
Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735343/
https://www.ncbi.nlm.nih.gov/pubmed/28824013
http://dx.doi.org/10.1088/2050-6120/aa872e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735343/
https://www.ncbi.nlm.nih.gov/pubmed/28824013
http://dx.doi.org/10.1088/2050-6120/aa872e

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