Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease

Site-specific recombination systems like those based on the Flp recombinase proved themselves as efficient tools for cell line engineering. The recent emergence of designer nucleases, especially RNA guided endonucleases like Cas9, has considerably broadened the available toolbox for applications lik...

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Detalles Bibliográficos
Autores principales: Phan, Quang Vinh, Contzen, Jörg, Seemann, Petra, Gossen, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736728/
https://www.ncbi.nlm.nih.gov/pubmed/29259215
http://dx.doi.org/10.1038/s41598-017-17651-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736728/
https://www.ncbi.nlm.nih.gov/pubmed/29259215
http://dx.doi.org/10.1038/s41598-017-17651-0

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