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In vitro transcription–translation using bacterial genome as a template to reconstitute intracellular profile

In vitro transcription–translation systems (TX–TL) can synthesize most of individual genes encoded in genomes by using strong promoters and translation initiation sequences. This fact raises a possibility that TX–TL using genome as a template can reconstitute the profile of RNA and proteins in livin...

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Detalles Bibliográficos
Autores principales: Fujiwara, Kei, Sawamura, Tsunehito, Niwa, Tatsuya, Deyama, Tatsuki, Nomura, Shin-ichiro M., Taguchi, Hideki, Doi, Nobuhide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737407/
https://www.ncbi.nlm.nih.gov/pubmed/28977538
http://dx.doi.org/10.1093/nar/gkx776