Cargando…
In vitro transcription–translation using bacterial genome as a template to reconstitute intracellular profile
In vitro transcription–translation systems (TX–TL) can synthesize most of individual genes encoded in genomes by using strong promoters and translation initiation sequences. This fact raises a possibility that TX–TL using genome as a template can reconstitute the profile of RNA and proteins in livin...
Autores principales: | Fujiwara, Kei, Sawamura, Tsunehito, Niwa, Tatsuya, Deyama, Tatsuki, Nomura, Shin-ichiro M., Taguchi, Hideki, Doi, Nobuhide |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737407/ https://www.ncbi.nlm.nih.gov/pubmed/28977538 http://dx.doi.org/10.1093/nar/gkx776 |
Ejemplares similares
-
Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system
por: Fujiwara, Kei, et al.
Publicado: (2013) -
Reconstitution of intracellular environments in vitro and in artificial cells
por: Fujiwara, Kei, et al.
Publicado: (2014) -
Engineered multiple translation initiation sites: a novel tool to enhance protein production in Bacillus licheniformis and other industrially relevant bacteria
por: Zhang, Manyu, et al.
Publicado: (2022) -
A predictive biophysical model of translational coupling to coordinate and control protein expression in bacterial operons
por: Tian, Tian, et al.
Publicado: (2015) -
CRISPR-RNAa: targeted activation of translation using dCas13 fusions to translation initiation factors
por: Otoupal, Peter B, et al.
Publicado: (2022)