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Balancing gene expression without library construction via a reusable sRNA pool

Balancing protein expression is critical when optimizing genetic systems. Typically, this requires library construction to vary the genetic parts controlling each gene, which can be expensive and time-consuming. Here, we develop sRNAs corresponding to 15nt ‘target’ sequences that can be inserted ups...

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Detalles Bibliográficos
Autores principales: Ghodasara, Amar, Voigt, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737548/
https://www.ncbi.nlm.nih.gov/pubmed/28609783
http://dx.doi.org/10.1093/nar/gkx530