Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage

RNA dependent DNA-polymerases, reverse transcriptases, are key enzymes for retroviruses and retroelements. Their fidelity, including indel generation, is significant for their use as reagents including for deep sequencing. Here, we report that certain RNA template structures and G-rich sequences, ah...

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Detalles Bibliográficos
Autores principales: Penno, Christophe, Kumari, Romika, Baranov, Pavel V., van Sinderen, Douwe, Atkins, John F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737552/
https://www.ncbi.nlm.nih.gov/pubmed/28973469
http://dx.doi.org/10.1093/nar/gkx689

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737552/
https://www.ncbi.nlm.nih.gov/pubmed/28973469
http://dx.doi.org/10.1093/nar/gkx689

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