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CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases
The restriction endonuclease CglI from Corynebacterium glutamicum recognizes an asymmetric 5′-GCCGC-3′ site and cleaves the DNA 7 and 6/7 nucleotides downstream on the top and bottom DNA strands, respectively, in an NTP-hydrolysis dependent reaction. CglI is composed of two different proteins: an en...
Autores principales: | Toliusis, Paulius, Zaremba, Mindaugas, Silanskas, Arunas, Szczelkun, Mark D., Siksnys, Virginijus |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737866/ https://www.ncbi.nlm.nih.gov/pubmed/28854738 http://dx.doi.org/10.1093/nar/gkx580 |
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