Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken

In recent years, avian influenza virus H9N2 undergoing antigenic drift represents a threat to poultry farming as well as public health. Current vaccines are restricted to inactivated vaccine strains and their related variants. In this study, a recombinant H9N2 (H9N2-TM) strain with a replaced H3 hem...

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Autores principales: Zhang, Yun, Wei, Ying, Liu, Kang, Huang, Mengjiao, Li, Ran, Wang, Yang, Liu, Qiliang, Zheng, Jing, Xue, Chunyi, Cao, Yongchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738434/
https://www.ncbi.nlm.nih.gov/pubmed/29263359
http://dx.doi.org/10.1038/s41598-017-18054-x
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author Zhang, Yun
Wei, Ying
Liu, Kang
Huang, Mengjiao
Li, Ran
Wang, Yang
Liu, Qiliang
Zheng, Jing
Xue, Chunyi
Cao, Yongchang
author_facet Zhang, Yun
Wei, Ying
Liu, Kang
Huang, Mengjiao
Li, Ran
Wang, Yang
Liu, Qiliang
Zheng, Jing
Xue, Chunyi
Cao, Yongchang
author_sort Zhang, Yun
collection PubMed
description In recent years, avian influenza virus H9N2 undergoing antigenic drift represents a threat to poultry farming as well as public health. Current vaccines are restricted to inactivated vaccine strains and their related variants. In this study, a recombinant H9N2 (H9N2-TM) strain with a replaced H3 hemagglutinin (HA) transmembrane (TM) domain was generated. Virus assembly and viral protein composition were not affected by the transmembrane domain replacement. Further, the recombinant TM-replaced H9N2-TM virus could provide better inter-clade protection in both mice and chickens against H9N2, suggesting that the H3-TM-replacement could be considered as a strategy to develop efficient subtype-specific H9N2 influenza vaccines.
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spelling pubmed-57384342017-12-22 Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken Zhang, Yun Wei, Ying Liu, Kang Huang, Mengjiao Li, Ran Wang, Yang Liu, Qiliang Zheng, Jing Xue, Chunyi Cao, Yongchang Sci Rep Article In recent years, avian influenza virus H9N2 undergoing antigenic drift represents a threat to poultry farming as well as public health. Current vaccines are restricted to inactivated vaccine strains and their related variants. In this study, a recombinant H9N2 (H9N2-TM) strain with a replaced H3 hemagglutinin (HA) transmembrane (TM) domain was generated. Virus assembly and viral protein composition were not affected by the transmembrane domain replacement. Further, the recombinant TM-replaced H9N2-TM virus could provide better inter-clade protection in both mice and chickens against H9N2, suggesting that the H3-TM-replacement could be considered as a strategy to develop efficient subtype-specific H9N2 influenza vaccines. Nature Publishing Group UK 2017-12-20 /pmc/articles/PMC5738434/ /pubmed/29263359 http://dx.doi.org/10.1038/s41598-017-18054-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Yun
Wei, Ying
Liu, Kang
Huang, Mengjiao
Li, Ran
Wang, Yang
Liu, Qiliang
Zheng, Jing
Xue, Chunyi
Cao, Yongchang
Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken
title Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken
title_full Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken
title_fullStr Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken
title_full_unstemmed Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken
title_short Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken
title_sort recombinant influenza h9n2 virus with a substitution of h3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738434/
https://www.ncbi.nlm.nih.gov/pubmed/29263359
http://dx.doi.org/10.1038/s41598-017-18054-x
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