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Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food
OBJECTIVES: In Ecuador, food products need to be labeled if exceeded 0.9% of transgenic content in whole products. For the detection of genetically modified organisms (GMOs), three DNA extraction methods were tested in 35 food products commercialized in Ecuador. Samples with positive amplification o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738804/ https://www.ncbi.nlm.nih.gov/pubmed/29262852 http://dx.doi.org/10.1186/s13104-017-3083-x |
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author | Pacheco Coello, Ricardo Pestana Justo, Jorge Factos Mendoza, Andrés Santos Ordoñez, Efrén |
author_facet | Pacheco Coello, Ricardo Pestana Justo, Jorge Factos Mendoza, Andrés Santos Ordoñez, Efrén |
author_sort | Pacheco Coello, Ricardo |
collection | PubMed |
description | OBJECTIVES: In Ecuador, food products need to be labeled if exceeded 0.9% of transgenic content in whole products. For the detection of genetically modified organisms (GMOs), three DNA extraction methods were tested in 35 food products commercialized in Ecuador. Samples with positive amplification of endogenous genes were screened for the presence of the Cauliflower mosaic virus 35S-promoter (P35S) and the nopaline synthase-terminator (Tnos). TaqMan™ probes were used for determination of transgenic content of the GTS 40-3-2 and MON810 events through quantitative PCR (qPCR). RESULTS: Twenty-six processed food samples were positive for the P35S alone and eight samples for the Tnos and P35S. Absolute qPCR results indicated that eleven samples were positive for GTS 40-3-2 specific event and two for MON810 specific event. A total of nine samples for events GTS 40-3-2 and MON810 exceeded the umbral allowed of transgenic content in the whole food product with the specific events. Different food products may require different DNA extraction protocols for GMO detection through PCR. Among the three methods tested, the DNeasy mericon food kit DNA extraction method obtained higher proportion of amplified endogenous genes through PCR. Finally, event-specific GMOs were detected in food products in Ecuador. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-017-3083-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5738804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57388042018-01-02 Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food Pacheco Coello, Ricardo Pestana Justo, Jorge Factos Mendoza, Andrés Santos Ordoñez, Efrén BMC Res Notes Research Note OBJECTIVES: In Ecuador, food products need to be labeled if exceeded 0.9% of transgenic content in whole products. For the detection of genetically modified organisms (GMOs), three DNA extraction methods were tested in 35 food products commercialized in Ecuador. Samples with positive amplification of endogenous genes were screened for the presence of the Cauliflower mosaic virus 35S-promoter (P35S) and the nopaline synthase-terminator (Tnos). TaqMan™ probes were used for determination of transgenic content of the GTS 40-3-2 and MON810 events through quantitative PCR (qPCR). RESULTS: Twenty-six processed food samples were positive for the P35S alone and eight samples for the Tnos and P35S. Absolute qPCR results indicated that eleven samples were positive for GTS 40-3-2 specific event and two for MON810 specific event. A total of nine samples for events GTS 40-3-2 and MON810 exceeded the umbral allowed of transgenic content in the whole food product with the specific events. Different food products may require different DNA extraction protocols for GMO detection through PCR. Among the three methods tested, the DNeasy mericon food kit DNA extraction method obtained higher proportion of amplified endogenous genes through PCR. Finally, event-specific GMOs were detected in food products in Ecuador. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-017-3083-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-20 /pmc/articles/PMC5738804/ /pubmed/29262852 http://dx.doi.org/10.1186/s13104-017-3083-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Pacheco Coello, Ricardo Pestana Justo, Jorge Factos Mendoza, Andrés Santos Ordoñez, Efrén Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food |
title | Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food |
title_full | Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food |
title_fullStr | Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food |
title_full_unstemmed | Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food |
title_short | Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food |
title_sort | comparison of three dna extraction methods for the detection and quantification of gmo in ecuadorian manufactured food |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738804/ https://www.ncbi.nlm.nih.gov/pubmed/29262852 http://dx.doi.org/10.1186/s13104-017-3083-x |
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