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A novel cell exclusion zone assay with a barrier made from room temperature vulcanizing silicone rubber

OBJECTIVE: To examine the usefulness of room temperature vulcanizing (RTV) silicone rubber as a barrier material for cell exclusion zone assays. METHODS: We created barriers using three types of RTV silicone rubber with differing viscosities. We then assessed the adherence of these barriers to cultu...

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Detalles Bibliográficos
Autores principales: Shiode, Yusuke, Morizane, Yuki, Matoba, Ryo, Hirano, Masayuki, Doi, Shinichiro, Toshima, Shinji, Araki, Ryoichi, Hosogi, Mika, Takahashi, Kosuke, Kanzaki, Yuki, Yonezawa, Tomoko, Shiraga, Fumio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739469/
https://www.ncbi.nlm.nih.gov/pubmed/29267369
http://dx.doi.org/10.1371/journal.pone.0190198