Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome

The nucleoid of Escherichia coli comprises DNA, nucleoid associated proteins (NAPs) and RNA, whose role is unclear. We found that lysing bacterial cells embedded in agarose plugs in the presence of RNases caused massive fragmentation of the chromosomal DNA. This RNase-induced chromosomal fragmentati...

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Detalles Bibliográficos
Autores principales: Khan, Sharik R., Kuzminov, Andrei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739488/
https://www.ncbi.nlm.nih.gov/pubmed/29267353
http://dx.doi.org/10.1371/journal.pone.0190177

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739488/
https://www.ncbi.nlm.nih.gov/pubmed/29267353
http://dx.doi.org/10.1371/journal.pone.0190177

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