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Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome
The nucleoid of Escherichia coli comprises DNA, nucleoid associated proteins (NAPs) and RNA, whose role is unclear. We found that lysing bacterial cells embedded in agarose plugs in the presence of RNases caused massive fragmentation of the chromosomal DNA. This RNase-induced chromosomal fragmentati...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739488/ https://www.ncbi.nlm.nih.gov/pubmed/29267353 http://dx.doi.org/10.1371/journal.pone.0190177 |