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Automated One-pot Radiosynthesis of [(11)C]S-adenosyl Methionine

BACKGROUND: Glycine N-methyltransferase is an enzyme overexpressed in some neo-plastic tissues. It catalyses the methylation of glycine using S-adenosyl methionine (SAM or AdoM-et) as substrate. SAM is involved in a great variety of biochemical processes, including transmeth-ylation reactions. Thus,...

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Detalles Bibliográficos
Autores principales: Zoppolo, Florencia, Porcal, Williams, Oliver, Patricia, Savio, Eduardo, Engler, Henry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740492/
https://www.ncbi.nlm.nih.gov/pubmed/28721805
http://dx.doi.org/10.2174/1874471010666170718171441
Descripción
Sumario:BACKGROUND: Glycine N-methyltransferase is an enzyme overexpressed in some neo-plastic tissues. It catalyses the methylation of glycine using S-adenosyl methionine (SAM or AdoM-et) as substrate. SAM is involved in a great variety of biochemical processes, including transmeth-ylation reactions. Thus, [11C]SAM could be used to evaluate transmethylation activity in tumours. The only method reported for [11C]SAM synthesis is an enzymatic process with several limitations. We propose a new chemical method to obtain [11C]SAM, through a one-pot synthesis. METHOD: The optimization of [11C]SAM synthesis was carried out in the automated TRACERlab® FX C Pro module. Different labelling conditions were performed varying methylating agent, precur-sor amount, temperature and reaction time. The compound was purified using a semi-preparative HPLC. Radiochemical stability, lipophilicity and plasma protein binding were evaluated. RESULTS: The optimum labelling conditions were [11C]CH3OTf as the methylating agent, 5 mg of precursor dissolved in formic acid at 60 ºC for 1 minute. [11C]SAM was obtained as a diastereomer-ic mixture. Three batches were produced and quality control was performed according to specifica-tions. [11C]SAM was stable in final formulation and in plasma. Log POCT obtained for [11C]SAM was (-2,01 ± 0,07) (n=4), and its value for plasma protein binding was low. CONCLUSION: A new chemical method to produce [11C]SAM was optimized. The radiotracer was ob-tained as a diastereomeric mixture with a 53:47 [(R,S)-isomer: (S,S)-isomer] ratio. The compound was within the quality control specifications. In vitro stability was verified. This compound is suita-ble to perform preclinical and clinical evaluations.