Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension

We develop a strategy for haplotype analysis of PCR products that contained two adjacent heterozygous loci using sequencing with specific primers, allele-specific primers, and ddNTP-blocked primers. To validate its feasibility, two sets of PCR products, including two adjacent heterozygous SNPs, UGT1...

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Detalles Bibliográficos
Autores principales: Wang, Liu, Xiao, Pengfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742430/
https://www.ncbi.nlm.nih.gov/pubmed/29376065
http://dx.doi.org/10.1155/2017/1397902

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742430/
https://www.ncbi.nlm.nih.gov/pubmed/29376065
http://dx.doi.org/10.1155/2017/1397902

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