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Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension
We develop a strategy for haplotype analysis of PCR products that contained two adjacent heterozygous loci using sequencing with specific primers, allele-specific primers, and ddNTP-blocked primers. To validate its feasibility, two sets of PCR products, including two adjacent heterozygous SNPs, UGT1...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Hindawi
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742430/ https://www.ncbi.nlm.nih.gov/pubmed/29376065 http://dx.doi.org/10.1155/2017/1397902 |