Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins

We report the silencing of CA1 mRNA in PC3 and MDA cells. The levels of mRNA coding CA1 protein in the knock‐down mRNA (CA1 siRNA) cells have been measured by RT‐PCR and were approximately 5% (PC3) and 20% (MDA‐MB‐231), respectively, of the level of control (Mock siRNA) used during silencing. In PC3...

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Detalles Bibliográficos
Autores principales: Vulic, Radivojka, Tyciakova, Silvia, Dubrovcakova, Maria, Skultety, Ludovit, Lakota, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742735/
https://www.ncbi.nlm.nih.gov/pubmed/28782909
http://dx.doi.org/10.1111/jcmm.13315
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author Vulic, Radivojka
Tyciakova, Silvia
Dubrovcakova, Maria
Skultety, Ludovit
Lakota, Jan
author_facet Vulic, Radivojka
Tyciakova, Silvia
Dubrovcakova, Maria
Skultety, Ludovit
Lakota, Jan
author_sort Vulic, Radivojka
collection PubMed
description We report the silencing of CA1 mRNA in PC3 and MDA cells. The levels of mRNA coding CA1 protein in the knock‐down mRNA (CA1 siRNA) cells have been measured by RT‐PCR and were approximately 5% (PC3) and 20% (MDA‐MB‐231), respectively, of the level of control (Mock siRNA) used during silencing. In PC3 and MDA‐MB‐231 cells, the mRNAs for COL1A1 and COL4A4 were up‐regulated. The mRNAs for CTHRC1, LAMC2, and WNT7B were not changed when compared to the control. The morphology of the cells during the treatments remained the same. On the Western blots, the lysate from the silenced cells showed lower levels of CA I as well.
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spelling pubmed-57427352018-01-04 Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins Vulic, Radivojka Tyciakova, Silvia Dubrovcakova, Maria Skultety, Ludovit Lakota, Jan J Cell Mol Med Short Communication We report the silencing of CA1 mRNA in PC3 and MDA cells. The levels of mRNA coding CA1 protein in the knock‐down mRNA (CA1 siRNA) cells have been measured by RT‐PCR and were approximately 5% (PC3) and 20% (MDA‐MB‐231), respectively, of the level of control (Mock siRNA) used during silencing. In PC3 and MDA‐MB‐231 cells, the mRNAs for COL1A1 and COL4A4 were up‐regulated. The mRNAs for CTHRC1, LAMC2, and WNT7B were not changed when compared to the control. The morphology of the cells during the treatments remained the same. On the Western blots, the lysate from the silenced cells showed lower levels of CA I as well. John Wiley and Sons Inc. 2017-08-07 2018-01 /pmc/articles/PMC5742735/ /pubmed/28782909 http://dx.doi.org/10.1111/jcmm.13315 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Vulic, Radivojka
Tyciakova, Silvia
Dubrovcakova, Maria
Skultety, Ludovit
Lakota, Jan
Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
title Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
title_full Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
title_fullStr Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
title_full_unstemmed Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
title_short Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
title_sort silencing of ca1 mrna in tumour cells does not change the gene expression of the extracellular matrix proteins
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742735/
https://www.ncbi.nlm.nih.gov/pubmed/28782909
http://dx.doi.org/10.1111/jcmm.13315
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