Cargando…

Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method

BACKGROUND: Human pluripotent stem cells (hPSCs) provide a promising cell source for ocular cell replacement therapy, but often lack standardized and xenogeneic-free culture and differentiation protocols. We aimed to develop a xeno- and feeder cell-free culture system for undifferentiated hPSCs alon...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hongisto, Heidi, Ilmarinen, Tanja, Vattulainen, Meri, Mikhailova, Alexandra, Skottman, Heli
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747074/ https://www.ncbi.nlm.nih.gov/pubmed/29284513 http://dx.doi.org/10.1186/s13287-017-0738-4

Ejemplares similares

Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential
por: Vattulainen, Meri, et al.
Publicado: (2019)

Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5(+) and ∆Np63α(+) cells with limbal cell characteristics and high wound healing capacity
por: Vattulainen, Meri, et al.
Publicado: (2021)

Small-Molecule Induction Promotes Corneal Epithelial Cell Differentiation from Human Induced Pluripotent Stem Cells
por: Mikhailova, Alexandra, et al.
Publicado: (2014)

Toward the defined and xeno-free differentiation of functional human pluripotent stem cell–derived retinal pigment epithelial cells
por: Vaajasaari, Hanna, et al.
Publicado: (2011)

Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions
por: Grönroos, Pyry, et al.
Publicado: (2021)

Cell maturation influences the ability of hESC-RPE to tolerate cellular stress
por: Viheriälä, Taina, et al.
Publicado: (2022)

Comparative proteomics reveals human pluripotent stem cell-derived limbal epithelial stem cells are similar to native ocular surface epithelial cells
por: Mikhailova, Alexandra, et al.
Publicado: (2015)

The Effect of Human and Mouse Fibroblast Feeder Cells on Cardiac Differentiation of Human Pluripotent Stem Cells
por: Pekkanen-Mattila, Mari, et al.
Publicado: (2012)

Differential Expression of Inflammasome-Related Genes in Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells with or without History of Age-Related Macular Degeneration
por: Hytti, Maria, et al.
Publicado: (2021)

Comparative proteomic analysis of human embryonic stem cell-derived and primary human retinal pigment epithelium
por: Hongisto, Heidi, et al.
Publicado: (2017)

Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions
por: MacArthur, Chad C., et al.
Publicado: (2012)

A Defined and Xeno-Free Culture Method Enabling the Establishment of Clinical-Grade Human Embryonic, Induced Pluripotent and Adipose Stem Cells
por: Rajala, Kristiina, et al.
Publicado: (2010)

Xeno- and Feeder-Free Differentiation of Human iPSCs to Trabecular Meshwork-Like Cells by Recombinant Cytokines
por: Wang, Wenyan, et al.
Publicado: (2021)

Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium-Role in Dead Cell Clearance and Inflammation
por: Szatmári-Tóth, Mária, et al.
Publicado: (2019)

Propagation of Human Embryonic Stem Cells on Human Amniotic Fluid Cells as Feeder Cells in Xeno-Free Culture Conditions
por: Jung, Juwon, et al.
Publicado: (2016)

The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy
por: Souralova, Tereza, et al.
Publicado: (2022)

Development of a Xeno-Free Feeder-Layer System from Human Umbilical Cord Mesenchymal Stem Cells for Prolonged Expansion of Human Induced Pluripotent Stem Cells in Culture
por: Zou, Qing, et al.
Publicado: (2016)

Human Induced Pluripotent Stem Cells on Autologous Feeders
por: Takahashi, Kazutoshi, et al.
Publicado: (2009)

Easy xeno-free and feeder-free method for isolating and growing limbal stromal and epithelial stem cells of the human cornea
por: Ghoubay-Benallaoua, Djida, et al.
Publicado: (2017)

Influence of feeder cells on transcriptomic analysis of pluripotent stem cells
por: Wan, Haifeng, et al.
Publicado: (2022)

Drug Flux Across RPE Cell Models: The Hunt for An Appropriate Outer Blood–Retinal Barrier Model for Use in Early Drug Discovery
por: Hellinen, Laura, et al.
Publicado: (2020)

Derivation of feeder-free human extended pluripotent stem cells
por: Zheng, Ran, et al.
Publicado: (2021)

Derivation of feeder-free human extended pluripotent stem cells
por: Zheng, Ran, et al.
Publicado: (2021)

Neural Stem Cells Achieve and Maintain Pluripotency without Feeder Cells
por: Choi, Hyun Woo, et al.
Publicado: (2011)

Retraction: Hellinen et al. Drug Flux across RPE Cell Models: The Hunt for an Appropriate Outer Blood–Retinal Barrier Model for Use in Early Drug Discovery. Pharmaceutics 2020, 12, 176
por: Hellinen, Laura, et al.
Publicado: (2022)

Methanol fixed feeder layers altered the pluripotency and metabolism of bovine pluripotent stem cells
por: Xu, Wenqiang, et al.
Publicado: (2022)

Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model
por: Liu, Zengping, et al.
Publicado: (2021)

Xeno-free induced pluripotent stem cell-derived neural progenitor cells for in vivo applications
por: Rust, Ruslan, et al.
Publicado: (2022)

Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions
por: Wang, Juan, et al.
Publicado: (2015)

Chemically defined and xeno-free culture condition for human extended pluripotent stem cells
por: Liu, Bei, et al.
Publicado: (2021)

Feeder cells treated with ethanol can be used to maintain self‐renewal and pluripotency of human pluripotent stem cells
por: Ren, Yahui, et al.
Publicado: (2023)

Small non-coding RNA landscape of extracellular vesicles from human stem cells
por: Kaur, Sippy, et al.
Publicado: (2018)

Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA
por: Warren, Luigi, et al.
Publicado: (2012)

Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells without Microenvironment of Feeder Cells
por: Lee, Seung-Won, et al.
Publicado: (2018)

Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity
por: Higuchi, Akon, et al.
Publicado: (2015)

A chemically-defined plastic scaffold for the xeno-free production of human pluripotent stem cells
por: Shimizu, Eiko, et al.
Publicado: (2022)

Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells
por: Isono, Wataru, et al.
Publicado: (2023)

Feeder Cells Support the Culture of Induced Pluripotent Stem Cells Even after Chemical Fixation
por: Yue, Xiao-Shan, et al.
Publicado: (2012)

Methanol fixed fibroblasts serve as feeder cells to maintain stem cells in the pluripotent state in vitro
por: Ren, Yahui, et al.
Publicado: (2018)

A Novel Feeder-Free Culture System for Human Pluripotent Stem Cell Culture and Induced Pluripotent Stem Cell Derivation
por: Vuoristo, Sanna, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_vnlf5g5d750iqevadfqg54riks