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Standardization and validation of real time PCR assays for the diagnosis of histoplasmosis using three molecular targets in an animal model

Histoplasmosis is considered one of the most important endemic and systemic mycoses worldwide. Until now few molecular techniques have been developed for its diagnosis. The aim of this study was to develop and evaluate three real time PCR (qPCR) protocols for different protein-coding genes (100-kDa,...

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Detalles Bibliográficos
Autores principales: López, Luisa F., Muñoz, César O., Cáceres, Diego H., Tobón, Ángela M., Loparev, Vladimir, Clay, Oliver, Chiller, Tom, Litvintseva, Anastasia, Gade, Lalitha, González, Ángel, Gómez, Beatriz L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747470/
https://www.ncbi.nlm.nih.gov/pubmed/29287097
http://dx.doi.org/10.1371/journal.pone.0190311