Cargando…
Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis
There is remarkable redundancy between the Cyclin–Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whe...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748970/ https://www.ncbi.nlm.nih.gov/pubmed/29074707 http://dx.doi.org/10.1083/jcb.201612147 |
_version_ | 1783289502235623424 |
---|---|
author | Strauss, Bernhard Harrison, Andrew Coelho, Paula Almeida Yata, Keiko Zernicka-Goetz, Magdalena Pines, Jonathon |
author_facet | Strauss, Bernhard Harrison, Andrew Coelho, Paula Almeida Yata, Keiko Zernicka-Goetz, Magdalena Pines, Jonathon |
author_sort | Strauss, Bernhard |
collection | PubMed |
description | There is remarkable redundancy between the Cyclin–Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whether it is required for cell division is contentious. Here, we used a novel imaging approach to analyze Cyclin B1–null embryos from fertilization onward. We show that Cyclin B1(−/−) embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1–Cdk1 at the plasma membrane precludes entry to mitosis. |
format | Online Article Text |
id | pubmed-5748970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57489702018-01-03 Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis Strauss, Bernhard Harrison, Andrew Coelho, Paula Almeida Yata, Keiko Zernicka-Goetz, Magdalena Pines, Jonathon J Cell Biol Research Articles There is remarkable redundancy between the Cyclin–Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whether it is required for cell division is contentious. Here, we used a novel imaging approach to analyze Cyclin B1–null embryos from fertilization onward. We show that Cyclin B1(−/−) embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1–Cdk1 at the plasma membrane precludes entry to mitosis. The Rockefeller University Press 2018-01-02 /pmc/articles/PMC5748970/ /pubmed/29074707 http://dx.doi.org/10.1083/jcb.201612147 Text en © 2018 Strauss et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Strauss, Bernhard Harrison, Andrew Coelho, Paula Almeida Yata, Keiko Zernicka-Goetz, Magdalena Pines, Jonathon Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
title | Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
title_full | Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
title_fullStr | Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
title_full_unstemmed | Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
title_short | Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
title_sort | cyclin b1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748970/ https://www.ncbi.nlm.nih.gov/pubmed/29074707 http://dx.doi.org/10.1083/jcb.201612147 |
work_keys_str_mv | AT straussbernhard cyclinb1isessentialformitosisinmouseembryosanditsnuclearexportsetsthetimeformitosis AT harrisonandrew cyclinb1isessentialformitosisinmouseembryosanditsnuclearexportsetsthetimeformitosis AT coelhopaulaalmeida cyclinb1isessentialformitosisinmouseembryosanditsnuclearexportsetsthetimeformitosis AT yatakeiko cyclinb1isessentialformitosisinmouseembryosanditsnuclearexportsetsthetimeformitosis AT zernickagoetzmagdalena cyclinb1isessentialformitosisinmouseembryosanditsnuclearexportsetsthetimeformitosis AT pinesjonathon cyclinb1isessentialformitosisinmouseembryosanditsnuclearexportsetsthetimeformitosis |