Cargando…

Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments

FRET biosensors have proven very useful tools for studying the activation of specific signalling pathways in living cells. Most biosensors designed to date have been predicated on fluorescent protein pairs that were identified by, and for use in, intensity based measurements, however fluorescence li...

Descripción completa

Detalles Bibliográficos
Autores principales: Martin, Kirsty J., McGhee, Ewan J., Schwarz, Juliana P., Drysdale, Martin, Brachmann, Saskia M., Stucke, Volker, Sansom, Owen J., Anderson, Kurt I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5749721/
https://www.ncbi.nlm.nih.gov/pubmed/29293509
http://dx.doi.org/10.1371/journal.pone.0183585