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In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

Mature oak (Quercus spp.) leaves, although abundantly available during the plants’ developmental cycle, are rarely exploited as viable sources of genomic DNA. These leaves are rich in metabolites difficult to remove during standard DNA purification, interfering with downstream molecular genetics app...

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Detalles Bibliográficos
Autores principales: Barta, Csengele E., Bolander, Bethany, Bilby, Steven R., Brown, Jeremy H., Brown, Reid N., Duryee, Alexander M., Edelman, Danielle R., Gray, Christina E., Gossett, Chandler, Haddock, Amie G., Helsel, Mackenzie M., Jones, Alyssa D., Klingseis, Marissa E., Leslie, Kalif, Miles, Edward W., Prawitz, Rachael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750628/
https://www.ncbi.nlm.nih.gov/pubmed/29073736
http://dx.doi.org/10.3390/plants6040052