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In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes
Mature oak (Quercus spp.) leaves, although abundantly available during the plants’ developmental cycle, are rarely exploited as viable sources of genomic DNA. These leaves are rich in metabolites difficult to remove during standard DNA purification, interfering with downstream molecular genetics app...
Autores principales: | Barta, Csengele E., Bolander, Bethany, Bilby, Steven R., Brown, Jeremy H., Brown, Reid N., Duryee, Alexander M., Edelman, Danielle R., Gray, Christina E., Gossett, Chandler, Haddock, Amie G., Helsel, Mackenzie M., Jones, Alyssa D., Klingseis, Marissa E., Leslie, Kalif, Miles, Edward W., Prawitz, Rachael A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5750628/ https://www.ncbi.nlm.nih.gov/pubmed/29073736 http://dx.doi.org/10.3390/plants6040052 |
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