Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Transposon mutagenesis is a method that allows gene disruption via the random genomic insertion of a piece of DNA called a transposon. The protocol below outlines a method for high efficiency transfer between bacterial strains of a plasmid harboring a transposon containing a kanamycin resistance mar...

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Detalles Bibliográficos
Autor principal: Freed, Nikki E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2017
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752320/
https://www.ncbi.nlm.nih.gov/pubmed/28994778
http://dx.doi.org/10.3791/56216

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752320/
https://www.ncbi.nlm.nih.gov/pubmed/28994778
http://dx.doi.org/10.3791/56216

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