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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
Transposon mutagenesis is a method that allows gene disruption via the random genomic insertion of a piece of DNA called a transposon. The protocol below outlines a method for high efficiency transfer between bacterial strains of a plasmid harboring a transposon containing a kanamycin resistance mar...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MyJove Corporation
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752320/ https://www.ncbi.nlm.nih.gov/pubmed/28994778 http://dx.doi.org/10.3791/56216 |