Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position

Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon base formation during protein synthesis. Our results suggest that codon:anticodon b...

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Detalles Bibliográficos
Autores principales: Liu, Wei, Shin, Dongwon, Ng, Martin, Sanbonmatsu, Karissa Y., Tor, Yitzhak, Cooperman, Barry S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753802/
https://www.ncbi.nlm.nih.gov/pubmed/28850078
http://dx.doi.org/10.3390/molecules22091427
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author Liu, Wei
Shin, Dongwon
Ng, Martin
Sanbonmatsu, Karissa Y.
Tor, Yitzhak
Cooperman, Barry S.
author_facet Liu, Wei
Shin, Dongwon
Ng, Martin
Sanbonmatsu, Karissa Y.
Tor, Yitzhak
Cooperman, Barry S.
author_sort Liu, Wei
collection PubMed
description Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5′- and 3′-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix.
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spelling pubmed-57538022018-01-04 Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position Liu, Wei Shin, Dongwon Ng, Martin Sanbonmatsu, Karissa Y. Tor, Yitzhak Cooperman, Barry S. Molecules Article Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5′- and 3′-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix. MDPI 2017-08-29 /pmc/articles/PMC5753802/ /pubmed/28850078 http://dx.doi.org/10.3390/molecules22091427 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Wei
Shin, Dongwon
Ng, Martin
Sanbonmatsu, Karissa Y.
Tor, Yitzhak
Cooperman, Barry S.
Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position
title Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position
title_full Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position
title_fullStr Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position
title_full_unstemmed Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position
title_short Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position
title_sort stringent nucleotide recognition by the ribosome at the middle codon position
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5753802/
https://www.ncbi.nlm.nih.gov/pubmed/28850078
http://dx.doi.org/10.3390/molecules22091427
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