Gaussian decomposition of high-resolution melt curve derivatives for measuring genome-editing efficiency

We describe a method for measuring genome editing efficiency from in silico analysis of high-resolution melt curve data. The melt curve data derived from amplicons of genome-edited or unmodified target sites were processed to remove the background fluorescent signal emanating from free fluorophore a...

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Detalles Bibliográficos
Autores principales: Zaboikin, Michail, Freter, Carl, Srinivasakumar, Narasimhachar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5754072/
https://www.ncbi.nlm.nih.gov/pubmed/29300734
http://dx.doi.org/10.1371/journal.pone.0190192

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5754072/
https://www.ncbi.nlm.nih.gov/pubmed/29300734
http://dx.doi.org/10.1371/journal.pone.0190192

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