Cargando…
Gaussian decomposition of high-resolution melt curve derivatives for measuring genome-editing efficiency
We describe a method for measuring genome editing efficiency from in silico analysis of high-resolution melt curve data. The melt curve data derived from amplicons of genome-edited or unmodified target sites were processed to remove the background fluorescent signal emanating from free fluorophore a...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2018
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5754072/ https://www.ncbi.nlm.nih.gov/pubmed/29300734 http://dx.doi.org/10.1371/journal.pone.0190192 |