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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
The popularity of the CRISPR/Cas9 system for both genome and epigenome engineering stems from its simplicity and adaptability. An effector (the Cas9 nuclease or a nuclease-dead dCas9 fusion protein) is targeted to a specific site in the genome by a small synthetic RNA known as the guide RNA, or gRNA...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MyJove Corporation
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755530/ https://www.ncbi.nlm.nih.gov/pubmed/29286403 http://dx.doi.org/10.3791/56264 |