The effect of DNA degradation bias in passive sampling devices on metabarcoding studies of arthropod communities and their associated microbiota

PCR amplification bias is a well-known problem in metagenomic analysis of arthropod communities. In contrast, variation of DNA degradation rates is a largely neglected source of bias. Differential degradation of DNA molecules could cause underrepresentation of taxa in a community sequencing sample....

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Detalles Bibliográficos
Autores principales: Krehenwinkel, Henrik, Fong, Marisa, Kennedy, Susan, Huang, Edward Greg, Noriyuki, Suzuki, Cayetano, Luis, Gillespie, Rosemary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755739/
https://www.ncbi.nlm.nih.gov/pubmed/29304124
http://dx.doi.org/10.1371/journal.pone.0189188

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755739/
https://www.ncbi.nlm.nih.gov/pubmed/29304124
http://dx.doi.org/10.1371/journal.pone.0189188

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