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The effect of DNA degradation bias in passive sampling devices on metabarcoding studies of arthropod communities and their associated microbiota
PCR amplification bias is a well-known problem in metagenomic analysis of arthropod communities. In contrast, variation of DNA degradation rates is a largely neglected source of bias. Differential degradation of DNA molecules could cause underrepresentation of taxa in a community sequencing sample....
Autores principales: | Krehenwinkel, Henrik, Fong, Marisa, Kennedy, Susan, Huang, Edward Greg, Noriyuki, Suzuki, Cayetano, Luis, Gillespie, Rosemary |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5755739/ https://www.ncbi.nlm.nih.gov/pubmed/29304124 http://dx.doi.org/10.1371/journal.pone.0189188 |
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