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Development of an in vitro pre-mRNA splicing assay using plant nuclear extract
BACKGROUND: Pre-mRNA splicing is an essential post-transcriptional process in all eukaryotes. In vitro splicing systems using nuclear or cytoplasmic extracts from mammalian cells, yeast, and Drosophila have provided a wealth of mechanistic insights into assembly and composition of the spliceosome, s...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757305/ https://www.ncbi.nlm.nih.gov/pubmed/29321806 http://dx.doi.org/10.1186/s13007-017-0271-6 |