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Development of an in vitro pre-mRNA splicing assay using plant nuclear extract

BACKGROUND: Pre-mRNA splicing is an essential post-transcriptional process in all eukaryotes. In vitro splicing systems using nuclear or cytoplasmic extracts from mammalian cells, yeast, and Drosophila have provided a wealth of mechanistic insights into assembly and composition of the spliceosome, s...

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Detalles Bibliográficos
Autores principales:	Albaqami, Mohammed, Reddy, Anireddy S. N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5757305/ https://www.ncbi.nlm.nih.gov/pubmed/29321806 http://dx.doi.org/10.1186/s13007-017-0271-6

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