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A novel one-step approach for the construction of yeast surface display Fab antibody libraries

BACKGROUND: Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as well as light chain display plasmids in differ...

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Detalles Bibliográficos
Autores principales: Rosowski, Simon, Becker, Stefan, Toleikis, Lars, Valldorf, Bernhard, Grzeschik, Julius, Demir, Deniz, Willenbücher, Iris, Gaa, Ramona, Kolmar, Harald, Zielonka, Stefan, Krah, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759264/
https://www.ncbi.nlm.nih.gov/pubmed/29316915
http://dx.doi.org/10.1186/s12934-017-0853-z