Cargando…

A novel one-step approach for the construction of yeast surface display Fab antibody libraries

BACKGROUND: Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as well as light chain display plasmids in differ...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rosowski, Simon, Becker, Stefan, Toleikis, Lars, Valldorf, Bernhard, Grzeschik, Julius, Demir, Deniz, Willenbücher, Iris, Gaa, Ramona, Kolmar, Harald, Zielonka, Stefan, Krah, Simon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759264/ https://www.ncbi.nlm.nih.gov/pubmed/29316915 http://dx.doi.org/10.1186/s12934-017-0853-z

Ejemplares similares

Selection of Antibodies with Tailored Properties by Application of High-Throughput Multiparameter Fluorescence-Activated Cell Sorting of Yeast-Displayed Immune Libraries
por: Schröter, Christian, et al.
Publicado: (2018)

Beyond bispecificity: Controlled Fab arm exchange for the generation of antibodies with multiple specificities
por: Yanakieva, Desislava, et al.
Publicado: (2022)

Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian Cells
por: Fiebig, David, et al.
Publicado: (2022)

REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast Cells
por: Rhiel, Laura, et al.
Publicado: (2014)

Grabbing the Bull by Both Horns: Bovine Ultralong CDR-H3 Paratopes Enable Engineering of ‘Almost Natural’ Common Light Chain Bispecific Antibodies Suitable For Effector Cell Redirection
por: Klewinghaus, Daniel, et al.
Publicado: (2022)

Dual Function pH Responsive Bispecific Antibodies for Tumor Targeting and Antigen Depletion in Plasma
por: Bogen, Jan P., et al.
Publicado: (2019)

Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platform
por: Pekar, Lukas, et al.
Publicado: (2020)

Specific Targeting of Lymphoma Cells Using Semisynthetic Anti-Idiotype Shark Antibodies
por: Macarrón Palacios, Arturo, et al.
Publicado: (2020)

Engineering IgG-Like Bispecific Antibodies—An Overview
por: Krah, Simon, et al.
Publicado: (2018)

Cattle-derived knob paratopes grafted onto peripheral loops of the IgG1 Fc region enable the generation of a novel symmetric bispecific antibody format
por: Yanakieva, Desislava, et al.
Publicado: (2023)

A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display
por: Schröter, Christian, et al.
Publicado: (2014)

Expeditious Generation of Biparatopic Common Light Chain Antibodies via Chicken Immunization and Yeast Display Screening
por: Bogen, Jan P., et al.
Publicado: (2020)

Structural insights and biomedical potential of IgNAR scaffolds from sharks
por: Zielonka, Stefan, et al.
Publicado: (2014)

Semi-synthetic vNAR libraries screened against therapeutic antibodies primarily deliver anti-idiotypic binders
por: Könning, Doreen, et al.
Publicado: (2017)

Milking the Cow: Cattle-Derived Chimeric Ultralong CDR-H3 Antibodies and Their Engineered CDR-H3-Only Knobbody Counterparts Targeting Epidermal Growth Factor Receptor Elicit Potent NK Cell-Mediated Cytotoxicity
por: Pekar, Lukas, et al.
Publicado: (2021)

Binding pocket stabilization by high-throughput screening of yeast display libraries
por: Lerma Romero, Jorge A., et al.
Publicado: (2022)

Generation and engineering of potent single domain antibody-based bispecific IL-18 mimetics resistant to IL-18BP decoy receptor inhibition
por: Lipinski, Britta, et al.
Publicado: (2023)

Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display
por: Könning, Doreen, et al.
Publicado: (2018)

Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library
por: Dantas-Barbosa, Carmela, et al.
Publicado: (2009)

Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection
por: Oloketuyi, Sandra, et al.
Publicado: (2021)

Mammalian display to secretion switchable libraries for antibody preselection and high throughput functional screening
por: Gaa, Ramona, et al.
Publicado: (2023)

TriTECM: A tetrafunctional T-cell engaging antibody with built-in risk mitigation of cytokine release syndrome
por: Carrara, Stefania C., et al.
Publicado: (2022)

Prompting Fab Yeast Surface Display Efficiency by ER Retention and Molecular Chaperon Co-expression
por: Mei, Meng, et al.
Publicado: (2019)

Selection and Characterization of YKL-40-Targeting Monoclonal Antibodies from Human Synthetic Fab Phage Display Libraries
por: Kang, Kyungjae, et al.
Publicado: (2020)

A library approach for the de novo high-throughput isolation of humanized VHH domains with favorable developability properties following camelid immunization
por: Arras, Paul, et al.
Publicado: (2023)

Greatest Hits—Innovative Technologies for High Throughput Identification of Bispecific Antibodies
por: Hofmann, Tim, et al.
Publicado: (2020)

Treating Bladder Cancer: Engineering of Current and Next Generation Antibody-, Fusion Protein-, mRNA-, Cell- and Viral-Based Therapeutics
por: Bogen, Jan P., et al.
Publicado: (2021)

Generation and analysis of the improved human HAL9/10 antibody phage display libraries
por: Kügler, Jonas, et al.
Publicado: (2015)

Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()
por: Schmaljohn, Connie, et al.
Publicado: (1999)

Production and Characterization of Novel Fabs Generated from Different Phage Display Libraries as Probes for Immunoassays for Gluten Detection in Food
por: Garcia-Calvo, Eduardo, et al.
Publicado: (2023)

Design of a Trispecific Checkpoint Inhibitor and Natural Killer Cell Engager Based on a 2 + 1 Common Light Chain Antibody Architecture
por: Bogen, Jan P., et al.
Publicado: (2021)

Recombinant Antibody Production Using a Dual-Promoter Single Plasmid System
por: Carrara, Stefania C., et al.
Publicado: (2021)

Targeted Phagocytosis Induction for Cancer Immunotherapy via Bispecific MerTK-Engaging Antibodies
por: Carrara, Stefania C., et al.
Publicado: (2022)

Bicistronic DNA display for in vitro selection of Fab fragments
por: Sumida, Takeshi, et al.
Publicado: (2009)

DNA Libraries for the Construction of Phage Libraries: Statistical and Structural Requirements and Synthetic Methods
por: Lindner, Thomas, et al.
Publicado: (2011)

Single chain Fab (scFab) fragment
por: Hust, Michael, et al.
Publicado: (2007)

A Generic Strategy to Generate Bifunctional Two-in-One Antibodies by Chicken Immunization
por: Harwardt, Julia, et al.
Publicado: (2022)

Generation of a symmetrical trispecific NK cell engager based on a two-in-one antibody
por: Harwardt, Julia, et al.
Publicado: (2023)

Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation
por: Chockalingam, Karuppiah, et al.
Publicado: (2020)

Generation and Characterization of Monoclonal Antibodies to the Ogawa Lipopolysaccharide of Vibrio cholerae O1 from Phage-Displayed Human Synthetic Fab Library
por: Kim, Dain, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_efv8lpk4rdt4lha0n2maamdhuu