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Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues

In this study, we have determined, using RSM (mixture design and Doehlert matrix), the optimum values of the independent variables to achieve the maximum response for the extraction of total phenolic compounds from Spondias mombin L bagasse agroindustrial residues in order to preserve their antioxid...

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Autores principales: Santos Felix, Antonio C., Novaes, Cleber G., Pires Rocha, Maísla, Barreto, George E., do Nascimento, Baraquizio B., Giraldez Alvarez, Lisandro D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760567/
https://www.ncbi.nlm.nih.gov/pubmed/29354632
http://dx.doi.org/10.3389/fchem.2017.00116
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author Santos Felix, Antonio C.
Novaes, Cleber G.
Pires Rocha, Maísla
Barreto, George E.
do Nascimento, Baraquizio B.
Giraldez Alvarez, Lisandro D.
author_facet Santos Felix, Antonio C.
Novaes, Cleber G.
Pires Rocha, Maísla
Barreto, George E.
do Nascimento, Baraquizio B.
Giraldez Alvarez, Lisandro D.
author_sort Santos Felix, Antonio C.
collection PubMed
description In this study, we have determined, using RSM (mixture design and Doehlert matrix), the optimum values of the independent variables to achieve the maximum response for the extraction of total phenolic compounds from Spondias mombin L bagasse agroindustrial residues in order to preserve their antioxidant activity. The extraction of phenolic compounds, as well as their antioxidant capacity and the capacity to scavenge ABTS, was determined by the modified DPPH method at different periods of time, temperature, velocity of rotation and solvents concentration. We observed that the optimum condition for the highest antioxidant yield was obtained using water (60.84%), acetone (30.31%), and ethanol (8.85%) at 30°C during 20 min at 50 rpm. We have also found that the maximum yield of total phenolics was 355.63 ± 9.77 (mg GAE/100 g), showing an EC(50) of 3,962.24 ± 41.20 (g fruit/g of DPPH) and 8.36 ± 0.30 (μM trolox/g fruit), which were measured using DPPH and ABTS assays. These results suggest that RSM was successfully applied for optimizing the extraction of phenolics compounds thus preserving their antioxidant activity.
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spelling pubmed-57605672018-01-19 Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues Santos Felix, Antonio C. Novaes, Cleber G. Pires Rocha, Maísla Barreto, George E. do Nascimento, Baraquizio B. Giraldez Alvarez, Lisandro D. Front Chem Chemistry In this study, we have determined, using RSM (mixture design and Doehlert matrix), the optimum values of the independent variables to achieve the maximum response for the extraction of total phenolic compounds from Spondias mombin L bagasse agroindustrial residues in order to preserve their antioxidant activity. The extraction of phenolic compounds, as well as their antioxidant capacity and the capacity to scavenge ABTS, was determined by the modified DPPH method at different periods of time, temperature, velocity of rotation and solvents concentration. We observed that the optimum condition for the highest antioxidant yield was obtained using water (60.84%), acetone (30.31%), and ethanol (8.85%) at 30°C during 20 min at 50 rpm. We have also found that the maximum yield of total phenolics was 355.63 ± 9.77 (mg GAE/100 g), showing an EC(50) of 3,962.24 ± 41.20 (g fruit/g of DPPH) and 8.36 ± 0.30 (μM trolox/g fruit), which were measured using DPPH and ABTS assays. These results suggest that RSM was successfully applied for optimizing the extraction of phenolics compounds thus preserving their antioxidant activity. Frontiers Media S.A. 2018-01-05 /pmc/articles/PMC5760567/ /pubmed/29354632 http://dx.doi.org/10.3389/fchem.2017.00116 Text en Copyright © 2018 Santos Felix, Novaes, Pires Rocha, Barreto, do Nascimento and Giraldez Alvarez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Santos Felix, Antonio C.
Novaes, Cleber G.
Pires Rocha, Maísla
Barreto, George E.
do Nascimento, Baraquizio B.
Giraldez Alvarez, Lisandro D.
Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues
title Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues
title_full Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues
title_fullStr Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues
title_full_unstemmed Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues
title_short Mixture Design and Doehlert Matrix for the Optimization of the Extraction of Phenolic Compounds from Spondias mombin L Apple Bagasse Agroindustrial Residues
title_sort mixture design and doehlert matrix for the optimization of the extraction of phenolic compounds from spondias mombin l apple bagasse agroindustrial residues
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760567/
https://www.ncbi.nlm.nih.gov/pubmed/29354632
http://dx.doi.org/10.3389/fchem.2017.00116
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