Copper interactions with DNA of chromatin and its role in neurodegenerative disorders

In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent....

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Detalles Bibliográficos
Autores principales: Govindaraju, M., Shekar, H.S., Sateesha, S.B., Vasudeva Raju, P., Sambasiva Rao, K.R., Rao, K.S.J., Rajamma, A.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760996/
https://www.ncbi.nlm.nih.gov/pubmed/29403839
http://dx.doi.org/10.1016/j.jpha.2013.03.003
Descripción
Sumario:In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent. Binding efficiency of Cu(2+) ions to DNA is increased in proportion to the degree of unwinding of the double helix induced by denaturation. Altered B-DNA conformation will alter the integrity of DNA which may affect the normal process of DNA replication and transcription. Copper induced DNA damage in the brain may cause neurotoxicity and the neuronal cell death and is implicated in Alzheimer's disease and other neurological disorders.

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