Copper interactions with DNA of chromatin and its role in neurodegenerative disorders

In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent....

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Autores principales: Govindaraju, M., Shekar, H.S., Sateesha, S.B., Vasudeva Raju, P., Sambasiva Rao, K.R., Rao, K.S.J., Rajamma, A.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760996/
https://www.ncbi.nlm.nih.gov/pubmed/29403839
http://dx.doi.org/10.1016/j.jpha.2013.03.003
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author Govindaraju, M.
Shekar, H.S.
Sateesha, S.B.
Vasudeva Raju, P.
Sambasiva Rao, K.R.
Rao, K.S.J.
Rajamma, A.J.
author_facet Govindaraju, M.
Shekar, H.S.
Sateesha, S.B.
Vasudeva Raju, P.
Sambasiva Rao, K.R.
Rao, K.S.J.
Rajamma, A.J.
author_sort Govindaraju, M.
collection PubMed
description In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent. Binding efficiency of Cu(2+) ions to DNA is increased in proportion to the degree of unwinding of the double helix induced by denaturation. Altered B-DNA conformation will alter the integrity of DNA which may affect the normal process of DNA replication and transcription. Copper induced DNA damage in the brain may cause neurotoxicity and the neuronal cell death and is implicated in Alzheimer's disease and other neurological disorders.
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spelling pubmed-57609962018-02-05 Copper interactions with DNA of chromatin and its role in neurodegenerative disorders Govindaraju, M. Shekar, H.S. Sateesha, S.B. Vasudeva Raju, P. Sambasiva Rao, K.R. Rao, K.S.J. Rajamma, A.J. J Pharm Anal Research Article In this study, we have demonstrated the conformational changes to DNA induced by abnormal interactions of copper using circular dichroism, in combination with UV-absorbance and fluorescence spectroscopy. Results confirm that binding of copper to bases of DNA in chromatin is concentration dependent. Binding efficiency of Cu(2+) ions to DNA is increased in proportion to the degree of unwinding of the double helix induced by denaturation. Altered B-DNA conformation will alter the integrity of DNA which may affect the normal process of DNA replication and transcription. Copper induced DNA damage in the brain may cause neurotoxicity and the neuronal cell death and is implicated in Alzheimer's disease and other neurological disorders. Xi'an Jiaotong University 2013-10 2013-04-28 /pmc/articles/PMC5760996/ /pubmed/29403839 http://dx.doi.org/10.1016/j.jpha.2013.03.003 Text en © 2013 Xi’an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Research Article
Govindaraju, M.
Shekar, H.S.
Sateesha, S.B.
Vasudeva Raju, P.
Sambasiva Rao, K.R.
Rao, K.S.J.
Rajamma, A.J.
Copper interactions with DNA of chromatin and its role in neurodegenerative disorders
title Copper interactions with DNA of chromatin and its role in neurodegenerative disorders
title_full Copper interactions with DNA of chromatin and its role in neurodegenerative disorders
title_fullStr Copper interactions with DNA of chromatin and its role in neurodegenerative disorders
title_full_unstemmed Copper interactions with DNA of chromatin and its role in neurodegenerative disorders
title_short Copper interactions with DNA of chromatin and its role in neurodegenerative disorders
title_sort copper interactions with dna of chromatin and its role in neurodegenerative disorders
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5760996/
https://www.ncbi.nlm.nih.gov/pubmed/29403839
http://dx.doi.org/10.1016/j.jpha.2013.03.003
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