Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells

Randomized mutagenesis at an endogenous chromosomal locus is a promising approach for protein engineering, functional assessment of regulatory elements, and modeling genetic variations. In mammalian cells, however, it is challenging to perform site-specific single-nucleotide substitution with single...

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Detalles Bibliográficos
Autores principales: Ishida, Kentaro, Xu, Huaigeng, Sasakawa, Noriko, Lung, Mandy Siu Yu, Kudryashev, Julia Alexandra, Gee, Peter, Hotta, Akitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762678/
https://www.ncbi.nlm.nih.gov/pubmed/29321585
http://dx.doi.org/10.1038/s41598-017-18568-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762678/
https://www.ncbi.nlm.nih.gov/pubmed/29321585
http://dx.doi.org/10.1038/s41598-017-18568-4

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